mouse anti cspg4  (Santa Cruz Biotechnology)

Journal: NPJ Regenerative Medicine

Article Title: Platelet derived growth factor-AB modulates post-infarct myocardium leading to extended improvement in cardiac function

doi: 10.1038/s41536-025-00433-y

Figure Lengend Snippet: Coronary artery pressure wire assessment was performed at baseline pre-infarct (D0) as well as D30 and D60 post-MI. We also employed immunohistochemistry (IHC) to assess infarct zone (IZ) arteriolar density, capillary density and NG2 + mural cell coverage. A Representative image of coronary pressure wire assessment. B Index of Microcirculatory Resistance (IMR). C Coronary Flow Reserve (CFR). D Representative image of NG2 + staining in control animal. E Representative image of NG2 + staining in PDGF-AB treated animal. F Arteriolar (aSMA + CD31 + vessels) density in IZ. G Capillary (CD31 + vessels) density in IZ. H – NG2 + capillary density in IZ I - Percentage of NG2 + cells in IZ. B , C Error bars = median±IQR, ( D – G ) error bars = mean ± SD; n = 5 in Control and PDGF-AB groups, n = 2 in Sham group; Two-way analysis of variance (ANOVA), followed by the Dunnett post hoc test to adjust for multiple comparisons, was performed for inter-group comparison of IMR and CFR; Mann-Whitney U test used for ( F − I ) ns – non-significant, ∗ P < 0.05, ∗∗ P < 0.01. Figure created and edited using GraphPad and Adobe Illustrator.

Article Snippet: The sections were blocked using commercial blocking buffer for 1 h at room temperature and then incubated in primary antibodies (NG2 (mouse; Santa Cruz Biotechnology #sc-166251)) at 1:200, aSMA-FITC (Sigma Aldrich #F3777-0.2 ml) at 1:400 and CD31 (rabbit; Abcam/Cat No. ab28364) at 1:50 overnight at 4 °C.

Techniques: Immunohistochemistry, Staining, Control, Comparison, MANN-WHITNEY